Injectable microcapillary network hydrogels engineered by liquid-liquid phase separation for stem cell transplantation

Akihiro Nishiguchi; Shima Ito; Kazuhiro Nagasaka; Hiyori Komatsu; Koichiro Uto; Tetsushi Taguchi

doi:10.1016/j.biomaterials.2023.122451

論文 Injectable microcapillary network hydrogels engineered by liquid-liquid phase separation for stem cell transplantation

Akihiro Nishiguchi (National Institute for Materials Science) ; Shima Ito (National Institute for Materials Science) ; Kazuhiro Nagasaka ; Hiyori Komatsu (National Institute for Materials Science) ; Koichiro Uto (National Institute for Materials Science) ; Tetsushi Taguchi (National Institute for Materials Science)

Manuscript for Biomaterials_final version.pdf

Download file (2.11MB)
Download as zip (2MB)

コレクション

引用

Akihiro Nishiguchi, Shima Ito, Kazuhiro Nagasaka, Hiyori Komatsu, Koichiro Uto, Tetsushi Taguchi. Injectable microcapillary network hydrogels engineered by liquid-liquid phase separation for stem cell transplantation. Biomaterials. 2023, 305 (), 122451. https://doi.org/10.1016/j.biomaterials.2023.122451

(BibTeX)

説明:

(abstract)

Injectable hydrogels are promising carriers for cell delivery in regenerative medicine. However, injectable hydrogels composed of crosslinked polymer networks are often non-porous and prevent biological communication with host tissues through signals, nutrients, oxygen, and cells, thereby limiting graft survival and tissue integration. Here we report injectable hydrogels with liquid-liquid phase separation-induced microcapillary networks (µCN) as stem cell-delivering scaffolds. The molecular modification of gelatin with hydrogen bonding moieties induced liquid-liquid phase separation when mixed with unmodified gelatin to form µCN structures in the hydrogels. Through spatiotemporally controlled covalent crosslinking and dissolution processes, porous µCN structures were formed in the hydrogels, which can enhance mass transport and cellular activity. The encapsulation of cells with injectable µCN hydrogels improved cellular adhesion, spreading, migration, and proliferation. Transplantation of mesenchymal stem cells with injectable µCN hydrogels enhanced graft survival and recovered hindlimb ischemia by enhancing material-tissue communication with biological signals and cells through µCN. This facile approach may serve as an advanced scaffold for improving stem cell transplantation therapies in regenerative medicine.

権利情報: